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Science Story

What is GC?(gas chromatography)

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1. GC (Gas Chromatography)
- An analytical technique to analyze mixture compounds




2. Strong & weak point of GC tech

Strong
- Easy technique
ex) put -> detect
- Basic tool in industries
- Quantity of the components

Weak
- Difficult to seperate similar B.P component
ex) cis Vs trans

3. How to work
- Injection in sample port -> vaporizing -> entering chromatographic column by the inert gas (Ar, He etc.) -> collecting signal by TCD, FID etc.

To determine the unknown components with concentration, external and internal standards are needed.

The peak retention time and area are compared to the known sample calibrated.

4. How to choose column
- depending on stationary phase, internal diameter, film thickness, lengh

ex) polarity, acid etc.

5. Brand
- Agilent: US, expensive but high quality
-Shimadzu: Japan, cheap, common trouble

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